Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome
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Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae
1
Recipe Chloramphenicol Stock Solution (10 mg/mL) 1
chloramphenicol (or 2 mL of a 3 mg/mL chloramphenicol stock solution) and 60 µL of Tween-80 to 200 mL of ultrapure water, heat to boil for 2-3 min with stirring
= 323
Applicability This method is suitable for the determination and confirmation of chloramphenicol in bovine muscle at levels ≥ 0
These dissolve much easier than the other preparations
Chloramphenicol : Cam : 20 mg/mL : 20 µg/mL : 1,000× : EtOH : link : Cycloheximide : Chx : 100 mg/mL : 100 µg/mL : 1,000× : 100% DMSO : link: Anti-fungal, toxic to
Due to
Chloramphenicol is a medication used in the management and treatment of superficial eye infections such as bacterial conjunctivitis, and otitis externa
com Stock Solution TD-S Revision 2
It is often used for bacterial selection in molecular biology applications at 10-20μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes
Preparation
Tip:We recommend using a 15 ml screw-capped tube with milliliter marks
Chloramphenicol Chloramphenicol, CAS 56-75-7, is a synthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes
The pH value at 25 °C is in the range of 5
Chloramphenicol has been used as a bacteriostatic non potent antibiotic
CT DG 18 Agar ISO 21527-2
72g of chloramphenicol sulfate into a small weight boat
5 – 151
C6455 chromatogram of the assay preparation corresponds to that in the standard preparation as obtained in the assay conforms to USP RS lot M Melting range 〈741〉 between 149° and 153°C 150-151°C Specific rotation, [α]D25 Chloramphenicol (40 mL) For a stock solution of 7
pH 7
Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae
Product name : Chloramphenicol Product Number : C0378 Brand : Sigma CAS-No
Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome
Sample preparation – liquid liquid extraction of phenicols in honey
Add 1 g of Chloramphenicol to the solution
Learn more about its composition, preparation and applications at MilliporeSigma